Metabolic engineering of phosphite metabolism in Synechococcus elongatus PCC 7942 as an effective measure to control biological contaminants in outdoor raceway ponds.
Sandra Isabel González-MoralesNavid Berenice Pacheco-GutiérrezCarlos A Ramírez-RodríguezAlethia A Brito-BelloPriscila Estrella-HernándezLuis Herrera-EstrellaDamar Lizbeth López-ArredondoPublished in: Biotechnology for biofuels (2020)
Our results suggest that the PtxD/phosphite system is a stable and sufficiently robust strategy to control biological contaminants without the need of sterilization or other complex aseptic procedures. Our data show that the PtxD/phosphite system can be used as selectable marker and allows production of the cyanobacterium S. elongatus PCC 7942 in non-axenic outdoor reactors at lower cost, which in principle should be applicable to other cyanobacteria and microalgae engineered to metabolize phosphite.