Neuroprotective Effects of Açaí (Euterpe oleracea Mart.) against Rotenone In Vitro Exposure.
Alencar Kolinski MachadoAna Cristina AndreazzaTatiane Morgana da SilvaAline Augusti BoligonVanusa do NascimentoGustavo ScolaAngela DuongFrancine Carla CadonáEuler Esteves RibeiroIvana Beatrice Mânica da CruzPublished in: Oxidative medicine and cellular longevity (2016)
Neuropsychiatric diseases, such as bipolar disorder (BD) and schizophrenia (SCZ), have a very complex pathophysiology. Several current studies describe an association between psychiatric illness and mitochondrial dysfunction and consequent cellular modifications, including lipid, protein, and DNA damage, caused by cellular oxidative stress. Euterpe oleracea (açaí) is a powerful antioxidant fruit. Açaí is an Amazonian palm fruit primarily found in the lowlands of the Amazonian rainforest, particularly in the floodplains of the Amazon River. Given this proposed association, this study analyzed the potential in vitro neuropharmacological effect of Euterpe oleracea (açaí) extract in the modulation of mitochondrial function and oxidative metabolism. SH-SY5Y cells were treated with rotenone to induce mitochondrial complex I dysfunction and before and after we exposed the cells to açaí extract at 5 μg/mL. Treated and untreated cells were then analyzed by spectrophotometric, fluorescent, immunological, and molecular assays. The results showed that açaí extract can potentially increase protein amount and enzyme activity of mitochondrial complex I, mainly through NDUFS7 and NDUFS8 overexpression. Açaí extract was also able to decrease cell reactive oxygen species levels and lipid peroxidation. We thus suggest açaí as a potential candidate for drug development and a possible alternative BD therapy.
Keyphrases
- oxidative stress
- induced apoptosis
- dna damage
- bipolar disorder
- cell cycle arrest
- ischemia reperfusion injury
- diabetic rats
- reactive oxygen species
- endoplasmic reticulum stress
- anti inflammatory
- cell death
- stem cells
- cell proliferation
- dna repair
- single molecule
- mass spectrometry
- cell therapy
- heat shock
- newly diagnosed
- quantum dots
- case control
- bone marrow
- heat stress
- tandem mass spectrometry
- liquid chromatography