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Molecular resolution imaging by repetitive optical selective exposure.

Lusheng GuYuanyuan LiShuwen ZhangYanhong XueWeixing LiDong LiTao XuWei Ji
Published in: Nature methods (2019)
We introduce an interferometric single-molecule localization method for super-resolution fluorescence microscopy. Fluorescence molecules are located by the intensities of multiple excitation patterns of an interference fringe, providing around a twofold improvement in the localization precision compared with the conventional imaging with the same photon budget. We demonstrate this technique by resolving nanostructures down to 5 nm in size over a large 25 × 25 μm2 field of view.
Keyphrases
  • single molecule
  • living cells
  • high resolution
  • atomic force microscopy
  • high frequency
  • photodynamic therapy
  • high speed
  • energy transfer
  • mass spectrometry