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The rapid-tome, a 3D-printed microtome, and an updated hand-sectioning method for high-quality plant sectioning.

David J ThomasJordan RainbowLaura E Bartley
Published in: Plant methods (2023)
The described hand-sectioning method is suitable for softer tissues, including hollow-stemmed grasses and similar samples. In addition, the Rapid-Tome provides capacity to safely produce high-quality sections of tougher plant materials at a fraction of the cost of traditional microtomes combined with excellent sample control. The Rapid-Tome features rapid sectioning, sample advancement, blade changes, and sample changes; it is highly portable and can be used easily with minimal training making production of thin sections accessible for classroom and outreach use, in addition to research.
Keyphrases
  • loop mediated isothermal amplification
  • gene expression
  • mass spectrometry
  • high resolution
  • low cost
  • quantum dots
  • molecularly imprinted