False-Positive Results for Human Immunodeficiency Virus Type 1 Nucleic Acid Amplification Testing in Chimeric Antigen Receptor T Cell Therapy.
Jocelyn R HauserHong HongN Esther BabadyGenovefa A PapanicolaouYi-Wei TangPublished in: Journal of clinical microbiology (2019)
Chimeric antigen receptor (CAR) T cell immunotherapy has been a major advancement in cancer therapeutics. Reprogramming of T cells is achieved by using gammaretroviral or lentiviral vectors, which may interfere with human immunodeficiency virus type 1 (HIV-1) nucleic acid amplification testing (NAAT). Here, we describe three clinical scenarios in which CAR T cell immunotherapy interfered with HIV-1 testing, including (i) routine infectious disease screening prior to stem cell transplantation in a 16-year-old female with B cell acute lymphoblastic leukemia, post CAR T cell treatment; (ii) routine infectious disease screening prior to second CAR T cell collection in a 65-year-old male with diffuse large B cell lymphoma who failed initial CAR T cell treatment; and (iii) routine infectious risk assessment following an occupational health exposure from a 58-year-old male with multiple myeloma, who received CAR T cell treatment. In each case, patients initially tested negative by the "fourth-generation" HIV-1 screening enzyme immunoassay (targeting the p24 antigen and anti-HIV-1 antibodies), but positive by the Roche Cobas AmpliPrep/Cobas TaqMan HIV-1 test v2.0 (targeting gag and the long terminal repeat [LTR]). These samples subsequently retested negative using the Abbott m2000 RealTime HIV-1 assay, which targets the integrase gene. These results indicated that cross-reactions between lentiviral vectors and LTR genomes targeted in the HIV-1 NAAT caused the HIV-1 NAAT false-positive results.
Keyphrases
- human immunodeficiency virus
- antiretroviral therapy
- hiv testing
- hiv positive
- hiv infected
- men who have sex with men
- hepatitis c virus
- nucleic acid
- hiv aids
- diffuse large b cell lymphoma
- risk assessment
- acute lymphoblastic leukemia
- stem cell transplantation
- healthcare
- infectious diseases
- public health
- squamous cell carcinoma
- multiple myeloma
- south africa
- clinical practice
- high dose
- mental health
- ejection fraction
- gene expression
- single cell
- acute myeloid leukemia
- epstein barr virus
- small molecule
- replacement therapy
- smoking cessation
- copy number
- prognostic factors
- heavy metals
- climate change
- high throughput
- stem cells
- lymph node metastasis