Pectic polysaccharide from Nelumbo nucifera leaves promotes intestinal antioxidant defense in vitro and in vivo.
Chao HuangXi PengDe-Jiang PangJuan LiBerit Smestad PaulsenFrode RiseYu-Long ChenZheng-Li ChenRen-Yong JiaLi-Xia LiXu SongLianqiang CheZhong-Qiong YinYuan-Feng ZouPublished in: Food & function (2021)
In this study, the Nelumbo nucifera leaf polysaccharide (NNLP) was isolated by hot water extraction and ethanol precipitation. DEAE anion exchange chromatography and gel filtration were further performed to obtained the purified fraction NNLP-I-I, the molecular weight of which was 16.4 kDa. The monosaccharide composition analysis and linkage units determination showed that the fraction NNLP-I-I was a pectic polysaccharide. In addition, the NMR spectra analysis revealed that NNLP-I-I mainly consisted of a homogalacturonan backbone and rhamnogalacturonan I, containing a long HG region and short RG-I region, with AG-II and 1-3 linked rhamnose as side chains. The biological studies demonstrated that NNLP-I-I displayed antioxidant properties through mediating the Nrf2-regulated intestinal cellular antioxidant defense, which could protect cultured intestinal cells from oxidative stress and improve the intestinal function of aged mice.
Keyphrases
- oxidative stress
- anti inflammatory
- mass spectrometry
- magnetic resonance
- single cell
- induced apoptosis
- quantum dots
- endothelial cells
- diabetic rats
- ischemia reperfusion injury
- signaling pathway
- density functional theory
- dna methylation
- gene expression
- metabolic syndrome
- endoplasmic reticulum stress
- genome wide
- tandem mass spectrometry
- heat shock protein
- liquid chromatography
- skeletal muscle
- solid phase extraction
- water soluble
- heat shock