Understanding Breast Cancers through Spatial and High-Resolution Visualization Using Imaging Technologies.
Haruko TakahashiDaisuke KawaharaYutaka KikuchiPublished in: Cancers (2022)
Breast cancer is the most common cancer affecting women worldwide. Although many analyses and treatments have traditionally targeted the breast cancer cells themselves, recent studies have focused on investigating entire cancer tissues, including breast cancer cells. To understand the structure of breast cancer tissues, including breast cancer cells, it is necessary to investigate the three-dimensional location of the cells and/or proteins comprising the tissues and to clarify the relationship between the three-dimensional structure and malignant transformation or metastasis of breast cancers. In this review, we aim to summarize the methods for analyzing the three-dimensional structure of breast cancer tissue, paying particular attention to the recent technological advances in the combination of the tissue-clearing method and optical three-dimensional imaging. We also aimed to identify the latest methods for exploring the relationship between the three-dimensional cell arrangement in breast cancer tissues and the gene expression of each cell. Finally, we aimed to describe the three-dimensional imaging features of breast cancer tissues using noninvasive photoacoustic imaging methods.
Keyphrases
- high resolution
- gene expression
- breast cancer cells
- single cell
- childhood cancer
- mass spectrometry
- type diabetes
- dna methylation
- breast cancer risk
- cell therapy
- stem cells
- oxidative stress
- high speed
- mesenchymal stem cells
- fluorescence imaging
- metabolic syndrome
- working memory
- pregnant women
- photodynamic therapy
- insulin resistance
- pregnancy outcomes