Simultaneous derivatization and extraction of amphetamine and methamphetamine using dispersive liquid-liquid microextraction prior to their analysis using GC-FID in creatine supplements.
Mahdi GolsanamlouMahboob NematiMohammad Reza Afshar MogaddamMir Ali FarazajdehPublished in: Analytical methods : advancing methods and applications (2023)
This study was focused on the development of a sensitive, reliable, and efficient extraction procedure for the determination of amphetamine and methamphetamine utilized in the adulteration of creatine sports supplements. The separation and detection of the analytes were conducted using the gas chromatography-flame ionization detection method. In this study, the analytes were extracted from a supplement powder into a proper solvent by sonication. Then, the extract was mixed with butyl chloroformate to obtain their butylated derivatives and then concentrated by a dispersive liquid-liquid microextraction procedure. The method was performed in a short time. Under optimized extraction conditions, a linear range of 2.01-500 ng g -1 was obtained by a coefficient of determination ≥0.996. Low detection (0.22 ng g -1 and 0.61 ng g -1 for amphetamine and methamphetamine, respectively) and quantification (0.73 ng g -1 and 2.01 ng g -1 for amphetamine and methamphetamine, respectively) limits, good precision (relative standard deviations ≤8.2%), and high extraction recoveries (79% and 86% for amphetamine and methamphetamine, respectively) were achieved. The usefulness of the method in the analysis of the target compounds was confirmed by studying the matrix effect and analysis of the analytes in different real samples.
Keyphrases
- gas chromatography
- solid phase extraction
- tandem mass spectrometry
- mass spectrometry
- gas chromatography mass spectrometry
- high resolution mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- ultra high performance liquid chromatography
- liquid chromatography tandem mass spectrometry
- molecularly imprinted
- simultaneous determination
- loop mediated isothermal amplification
- ionic liquid
- real time pcr
- magnetic resonance imaging
- high resolution
- anti inflammatory
- ms ms