Enhanced Imaging of Specific Cell-Surface Glycosylation Based on Multi-FRET.
Baoyin YuanYuanyuan ChenYuqiong SunQiuping GuoJin HuangJianbo LiuXiangxian MengXiaohai YangXiaohong WenZenghui LiLie LiKemin WangPublished in: Analytical chemistry (2018)
Cell-surface glycosylation contains abundant biological information that reflects cell physiological state, and it is of great value to image cell-surface glycosylation to elucidate its functions. Here we present a hybridization chain reaction (HCR)-based multifluorescence resonance energy transfer (multi-FRET) method for specific imaging of cell-surface glycosylation. By installing donors through metabolic glycan labeling and acceptors through aptamer-tethered nanoassemblies on the same glycoconjugate, intramolecular multi-FRET occurs due to near donor-acceptor distance. Benefiting from amplified effect and spatial flexibility of the HCR nanoassemblies, enhanced multi-FRET imaging of specific cell-surface glycosylation can be obtained. With this HCR-based multi-FRET method, we achieved obvious contrast in imaging of protein-specific GalNAcylation on 7211 cell surfaces. In addition, we demonstrated the general applicability of this method by visualizing the protein-specific sialylation on CEM cell surfaces. Furthermore, the expression changes of CEM cell-surface protein-specific sialylation under drug treatment was accurately monitored. This developed imaging method may provide a powerful tool in researching glycosylation functions, discovering biomarkers, and screening drugs.
Keyphrases
- cell surface
- energy transfer
- single molecule
- high resolution
- living cells
- quantum dots
- single cell
- healthcare
- gold nanoparticles
- magnetic resonance
- binding protein
- deep learning
- magnetic resonance imaging
- computed tomography
- cystic fibrosis
- mesenchymal stem cells
- mass spectrometry
- pseudomonas aeruginosa
- amino acid
- sensitive detection
- bone marrow
- kidney transplantation
- electronic health record
- combination therapy