A genetically encoded sensor for measuring serotonin dynamics.

Jin-Xia WanWanling PengXuelin LiTongrui Qian Kun Song Jianzhi ZengFei DengSuyu HaoJiesi FengPeng ZhangYajun ZhangJing ZouSunlei PanMimi ShinB Jill VentonJ Julius ZhuMiao JingMin Xu Yu-Long Li

Published in: Nature neuroscience (2021)

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.

Keyphrases

multiple sclerosis
resting state
metabolic syndrome
skeletal muscle
functional connectivity
brain injury
high fat diet induced
structural basis