Highly Antifungal Activity of Biosynthesized Copper Oxide Nanoparticles against Candida albicans .
Luis Enrique Garcia-MarinKarla Juarez-MorenoAlfredo Rafael Vilchis NestorErnestina Castro-LongoriaPublished in: Nanomaterials (Basel, Switzerland) (2022)
Candida albicans (ATCC SC5314) was exposed to biosynthesized copper oxide nanoparticles (CuONPs) to determine their inhibitory capacity. Nanoparticles were polydisperse of small size (5.8 ± 3.5 nm) with irregular shape. The minimum inhibitory concentration (MIC) against C. albicans was 35.5 µg/mL. The production of reactive oxygen species (ROS) of C. albicans was verified when exposed to different concentrations of CuONPs. Ultrastructural analysis of C. albicans revealed a high concentration of CuONPs in the cytoplasm and outside the cell; also, nanoparticles were detected within the cell wall. Cytotoxic analyses using fibroblasts (L929), macrophages (RAW 264.7), and breast (MCF-12) cell lines show good results of cell viability when exposed at the MIC. Additionally, a hemocompatibility analysis was carried out and was found to be below 5%, considered the threshold for biocompatibility. Therefore, it is concluded that the biosynthesized CuONPs have a high potential for developing a topical antifungal treatment.
Keyphrases
- candida albicans
- oxide nanoparticles
- reactive oxygen species
- cell wall
- biofilm formation
- silver nanoparticles
- single cell
- cell death
- photodynamic therapy
- dna damage
- breast cancer cells
- cell therapy
- stem cells
- oxidative stress
- pseudomonas aeruginosa
- extracellular matrix
- combination therapy
- bone marrow
- staphylococcus aureus
- risk assessment
- wound healing
- anti inflammatory