Visualizing fungicide action: an in vivo tool for rapid validation of fungicides with target location HOG pathway.

Here, we developed a rapid in vivo tool for fluorescent-based validation of fungicides targeting the HOG-signaling pathway. Furthermore, using the fluorescent mutants generated in this study, we are able to visualize that fungicide action is dependent on the histidine kinase MoHik1p but operates in a different mechanism of pathway activation compared to osmotic stress. © 2018 Society of Chemical Industry.