Accelerated directed evolution of dye-decolorizing peroxidase using a bacterial extracellular protein secretion system (BENNY).
Abdulrahman H A AlessaKang Lan TeeDavid Gonzalez-PerezHossam E M Omar AliCaroline A EvansAlex TrevaskisJian-He XuTuck Seng WongPublished in: Bioresources and bioprocessing (2019)
To our best knowledge, this is the first report of applying OsmY to simplify the directed evolution workflow and to direct the extracellular secretion of a haem protein such as DyP4.