Structural, biocompatibility, and antibacterial properties of Ge-DLC nanocomposite for biomedical applications.
Mehmet Enes ArslanMustafa Şükrü KurtNaim AslanAbdurrahim KadiSena ÖnerŞeymanur ÇobanoğluAyşenur YaziciPublished in: Journal of biomedical materials research. Part B, Applied biomaterials (2022)
Integrative production of new nanocomposites has been used to enhance favorable features of biomaterials for unlocking ultimate potential of different molecules. In the present study, advantageous properties of diamond like carbons (DLC) and germanium (Ge) like greater biocompatibility and antibacterial attributes were aimed to combined into a thin film. For this purpose, 400 nm DLC-Ge nanocomposite was coated on the borosilicate glasses via the magnetron sputtering and surface characteristics was analyzed by scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and The Raman spectrum. Biocompatibility analysis were performed by 3-(4,5-Dimethylthiazol-2-yl) (MTT) cell viability assay and Hoechst 33258 fluorescent staining genotoxicity assessments on the human fibroblast cell line (HDFa). Finally, antibacterial properties of DLC-Ge nanocomposite coatings were investigated by Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923) bacterial attachment analysis. As a result of magnetron sputtering coating, nearly 400 nm thick DLC-Ge nanocomposite film showed a smooth, a non-porous, and a dense characteristic. Cell viability analysis showed that Ge-DLC coatings permits %95 cell surface growth of fibroblast cells. Also, there were no significant difference in aspect of nuclear abnormalities compared to the (-) control which showed nonmutagenic features of the thin film. Finally, antibacterial attachment analysis put forth that Ge-DLC coatings inhibits bacterial adhesion as %40 and %25 rates for P. aeruginosa and S. aureus bacterial strains, respectively. From these results, DLC-Ge nanocomposites could be proposed as a potential new biomaterial for various biomedical applications.
Keyphrases
- reduced graphene oxide
- staphylococcus aureus
- pseudomonas aeruginosa
- quantum dots
- electron microscopy
- photodynamic therapy
- carbon nanotubes
- solid phase extraction
- induced apoptosis
- anti inflammatory
- gold nanoparticles
- cell proliferation
- signaling pathway
- highly efficient
- risk assessment
- room temperature
- pi k akt
- cell death
- endoplasmic reticulum stress
- visible light
- human health
- single cell
- induced pluripotent stem cells
- light emitting