Facile synthesis of sulfur and oxygen co-doped graphitic carbon nitride quantum dots for on-off detection of Cu 2+ in real samples and living cells.
Mengting ZhangYulu ZhangMingyu GanLiping XieJing WangWeihua JiaWei BianShaomin ShuangMartin M F ChoiPublished in: Methods and applications in fluorescence (2022)
A fluorescent sulfur and oxygen co-doped graphitic carbon nitride quantum dots (S,O-CNQDs) were prepared from ethylenediaminetetraacetic acid disodium salt dihydrate and thiourea as the carbon and sulfur sources. The morphology and surface functional groups of S,O-CNQDs were characterized by X-ray diffraction, transmission electron microscopy, X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The fluorescence of S,O-CNQDs could be quenched efficiently by Cu 2+ under the optimum conditions. The S,O-CNQDs could function as an excellent fluorescent probe for Cu 2+ detection with a wide linear range of 0.50-15 μ M and a low detection limit of 0.58 nM. In addition, this fluorescent probe was employed for monitoring Cu 2+ in samples of tap water, lake water, human serum and urine with good recoveries from 99.0% to 110.0%. Moreover, the S,O-CNQDs with high cell penetration and low cytotoxicity were utilized for Cu 2+ detection in living cells. Owing to the excellent properties of S,O-CNQDs, the as-prepared S,O-CNQDs can be a potential candidate for biological applications.
Keyphrases
- living cells
- fluorescent probe
- quantum dots
- single molecule
- electron microscopy
- loop mediated isothermal amplification
- sensitive detection
- label free
- metal organic framework
- visible light
- energy transfer
- high resolution
- real time pcr
- magnetic resonance imaging
- photodynamic therapy
- dual energy
- climate change
- drinking water
- cell therapy
- bone marrow
- crystal structure