Ultrasound and high hydrostatic pressure extractions on antioxidant capacity, antiproliferative and apoptosis effects in gastric cancer cells by lemon extract treatment.
Claudia Giovagnoli-VicuñaVilbett Briones-LabarcaGiuliano BernalErwin de la Fuente-OrtegaJohan MacuerPublished in: Natural product research (2021)
The objective of the present study was to investigate the antioxidant and antiproliferative effects of lemon extracts (LE) against human gastric cancer cells (SNU-1) and normal human gastric epithelial cells (GES-1), and their mechanism of inducing cellular death. LE was obtained by three extraction methods: conventional (CE), ultrasound (UAE) and high hydrostatic pressure (HHPE). Experimental results show that the extraction method used to obtain the LE had a significant influence on antioxidant capacity, cellular proliferation, and induction of apoptosis in SNU-1 cells. LE was showed to be cytotoxic with an IC50 of 1747.0 µg mL-1 and 1704.3 µg mL-1, as obtained by UAE and HHPE, respectively. In addition, this extract induces apoptosis as evidenced by confocal microscopy. Our findings indicate that LE would be an attractive source of bioactive compounds with antioxidant and antiproliferative capacity and can improve the functional value of food.
Keyphrases
- oxidative stress
- cell cycle arrest
- induced apoptosis
- endothelial cells
- anti inflammatory
- endoplasmic reticulum stress
- cell death
- induced pluripotent stem cells
- magnetic resonance imaging
- pi k akt
- signaling pathway
- ultrasound guided
- combination therapy
- risk assessment
- computed tomography
- human health
- smoking cessation