Leveraging selective knockdown of Sost gene by polyethyleneimine-siRNA-chitosan reduced gold nanoparticles to promote osteogenesis in MC3T3-E1 & MEF cells.
Karishma NiveriaMohammed ZafaryabLargee BiswasAsiya MahtabAnita Kamra VermaPublished in: Nanomedicine (London, England) (2024)
Aim: Osteoporosis is a systemic skeletal disorder characterized by reduced osteoblast differentiation, predominantly by overexpression of the Sost gene. A layer-by-layer approach enabled encapsulation of Sost siRNA to enhance the short half-life and poor transfection capacity of siRNA. Materials & methods: Polyethyleneimine and siRNA on chitosan-coated gold nanoparticles (PEI/siRNA/Cs-AuNPs) were engineered using chitosan-reduced gold nanoparticles. They were characterized by dynamic light scattering, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared and gel-mobility assays. Detailed in vitro experiments, gene silencing and western blots were performed. Results: A total of 80% knockdown of the target sclerostin protein was observed by PEI/siRNA/Cs-AuNPs, q-PCR showed threefold downregulation of the Sost gene. Osteogenic markers RunX2 and Alp were significantly upregulated. Conclusion: We report a safe, biocompatible nanotherapeutic strategy to enhance siRNA protection and subsequent silencing to augment bone formation.
Keyphrases
- gold nanoparticles
- electron microscopy
- hyaluronic acid
- cancer therapy
- drug delivery
- reduced graphene oxide
- copy number
- cell proliferation
- transcription factor
- wound healing
- induced apoptosis
- signaling pathway
- mesenchymal stem cells
- bone marrow
- dna methylation
- gene expression
- small molecule
- binding protein
- body composition
- drug induced
- drug release