Multifunctional-high resolution imaging plate based on hydrophilic graphene for digital pathology.
Geonhee LeeYuna OhJung Tae NamSeulgi JiA-Rang JangDu Won JeongMinSoung KangSun Sook LeeSoosang ChaeDonghwi ChoJun Yeon HwangKyungeun LeeJeong-O LeePublished in: Nanotechnology (2022)
In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improved z -axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate.
Keyphrases
- high resolution
- room temperature
- carbon nanotubes
- electron microscopy
- walled carbon nanotubes
- fluorescence imaging
- mass spectrometry
- drug delivery
- single molecule
- ionic liquid
- machine learning
- high speed
- cancer therapy
- magnetic resonance
- molecular dynamics
- single cell
- optical coherence tomography
- ultrasound guided
- fine needle aspiration
- molecularly imprinted