A scalable serology solution for profiling humoral immune responses to SARS-CoV-2 infection and vaccination.
Karen ColwillYannick GalipeauMatthew StuibleChristian GervaisCorey ArnoldBhavisha RathodKento T AbeJenny H WangAdrian PasculescuMariam MaltsevaLynda RocheleauMartin PelchatMahya Fazel-ZarandiMariam IskilovaMiriam Barrios-RodilesLinda BennettKevin YauFrançois CholetteChristine MesaAngel X LiAimee PatersonMichelle A HladunewichPamela J GoodwinJeffrey L WranaSteven J DrewsSamira MubarekaAllison J McGeerJohn KimMarc-André LangloisAnne-Claude GingrasYves DurocherPublished in: Clinical & translational immunology (2022)
Measuring antibodies to three viral antigens and identify neutralising antibodies capable of disrupting spike-ACE2 interactions in high-throughput enables large-scale analyses of humoral immune responses to SARS-CoV-2 infection and vaccination. The reagents are available to enable scaling up of standardised serological assays, permitting inter-laboratory data comparison and aggregation.