Photo-editable macromolecular information.
Niklas Felix KönigAbdelaziz Al OuahabiLaurence OswaldRoza SzwedaLaurence CharlesJean-François LutzPublished in: Nature communications (2019)
Light-induced alteration of macromolecular information plays a central role in biology and is known to influence health, aging and Darwinian evolution. Here, we report that light can also trigger sequence variations in abiotic information-containing polymers. Sequence-coded poly(phosphodiester)s were synthesized using four phosphoramidite monomers containing either photo-sensitive or photo-inert substituents. These monomers allow different sequence manipulations. For instance, using two light-cleavable monomers containing o-nitrobenzyl ether and o-nitroveratryl ether motifs, photo-erasable digital polymers were prepared. These polymers can be decoded by tandem mass spectrometry but become unreadable after UVA exposure. The opposite behavior, i.e. photo-revealable sequences, was obtained with polymers made of two isobaric monomers containing light-cleavable o-nitrobenzyl ether and light-inert p-nitrobenzyl ether substituents. Furthermore, when the latter two monomers were used in conjunction with a third monomer bearing a light-inert OH group, site-directed photo-mutations were induced in synthetic polymers. This was used herein to change the meaning of binary sequences.
Keyphrases
- high glucose
- endothelial cells
- tandem mass spectrometry
- ionic liquid
- health information
- electron transfer
- public health
- high performance liquid chromatography
- liquid chromatography
- ultra high performance liquid chromatography
- mental health
- simultaneous determination
- high resolution
- amino acid
- risk assessment
- mass spectrometry
- transcription factor
- genetic diversity
- high resolution mass spectrometry