Sanzi Yangqin Decoction Alleviates Allergic Asthma by Modulating Th1/Th2 Balance: Coupling Network Pharmacology with Biochemical Pharmacology.
Wenpan PengYong XuYunhai ZhouJuanjuan WuGuiqing PengYanlan GuXian-Mei ZhouMingzhi PuPublished in: Evidence-based complementary and alternative medicine : eCAM (2022)
This study aimed to verify that Sanzi Yangqin Decoction (SYD) can relieve asthma in mice and explore the effect on TH1/Th2 balance. The targets of SYD and asthma were explored from the public database using various methods. The potential targets and signaling pathways were identified by KEGG enrichment analysis from DAVID database. Mice asthma models were established using OVA and aluminum hydroxide. Lung tissues of mice were stained with HE and Masson. The contents of IFN- γ , IL-4, and TNF- α in BALF and IgE in mouse serum were detected using ELISA. In addition, the changes in Th1 and Th2 cells of the spleen were detected by flow cytometry. Fourteen core targets including IL4, IFNG, and MMP9 were identified for the treatment of asthma by SYD. The content of IL-4 in the lung tissue and BALF was gradually decreased with the increase in SYD concentration, while the IFN- γ was gradually increased. The drug significantly reduced IgE levels in serum and TNF- α in BALF. The number of Th1 cells in the spleen increased, while Th2 cells decreased in a concentration-dependent manner. SYD can alleviate pulmonary inflammation, restore Th1/Th2 balance, and relieve asthma.
Keyphrases
- chronic obstructive pulmonary disease
- lung function
- induced apoptosis
- allergic rhinitis
- cell cycle arrest
- signaling pathway
- oxidative stress
- rheumatoid arthritis
- healthcare
- gene expression
- cell death
- adverse drug
- high fat diet induced
- emergency department
- dendritic cells
- cystic fibrosis
- air pollution
- pulmonary hypertension
- pi k akt
- type diabetes
- gold nanoparticles
- adipose tissue
- risk assessment
- mouse model
- insulin resistance
- skeletal muscle
- wild type
- smoking cessation