Simple, sensitive, and cost-effective detection of wAlbB Wolbachia in Aedes mosquitoes, using loop mediated isothermal amplification combined with the electrochemical biosensing method.
Parinda ThayanukulBenchaporn LertanantawongWorachart SirawarapornSurat CharasmongkolcharoenThanyarat ChaibunRattanalak JittungdeePattamaporn KittayapongPublished in: PLoS neglected tropical diseases (2022)
Our results suggest that both LAMP and BIOSENSOR, either used in combination or stand-alone, are robust and sensitive. The methods have good potential for routine detection of Wolbachia in mosquitoes during field surveillance and monitoring of Wolbachia-based release programs, especially in countries with limited resources.