PML Regulates the Epidermal Differentiation Complex and Skin Morphogenesis during Mouse Embryogenesis.
Anna PołećAlexander D RowePernille BlicherRajikala SuganthanMagnar BjøråsStig Ove BøePublished in: Genes (2020)
The promyelocytic leukemia (PML) protein is an essential component of nuclear compartments called PML bodies. This protein participates in several cellular processes, including growth control, senescence, apoptosis, and differentiation. Previous studies have suggested that PML regulates gene expression at a subset of loci through a function in chromatin remodeling. Here we have studied global gene expression patterns in mouse embryonic skin derived from Pml depleted and wild type mouse embryos. Differential gene expression analysis at different developmental stages revealed a key role of PML in regulating genes involved in epidermal stratification. In particular, we observed dysregulation of the late cornified envelope gene cluster, which is a sub-region of the epidermal differentiation complex. In agreement with these data, PML body numbers are elevated in basal keratinocytes during embryogenesis, and we observed reduced epidermal thickness and defective hair follicle development in PML depleted mouse embryos.
Keyphrases
- gene expression
- wound healing
- genome wide
- genome wide identification
- dna methylation
- dna damage
- oxidative stress
- wild type
- soft tissue
- acute myeloid leukemia
- bone marrow
- small molecule
- optical coherence tomography
- transcription factor
- cell proliferation
- endoplasmic reticulum stress
- electronic health record
- big data
- endothelial cells
- signaling pathway
- artificial intelligence
- binding protein
- data analysis
- pi k akt