Spatial transcriptomics reveals unique gene expression changes in different brain regions after sleep deprivation.

Spatial transcriptomics using the Visium platform reveals the transcriptional signature across the brain, recapitulating the anatomy of the mouse brainSleep deprivation induces transcriptomic changes unique to each brain regionThe hippocampus is the brain region impacted the most by acute sleep deprivation, with most differentially regulated genes significantly downregulatedThe neocortex exhibits layer-specific changes in gene expression, with most differentially regulated genes significantly upregulatedRegistration of spatial transcriptomic data to a common anatomical reference space (Allen Common Coordinate Framework) allows statistical analysis of gene expression across regions of the brain and for multi-sample analysis.