Engineering of CYP76AH15 can improve activity and specificity towards forskolin biosynthesis in yeast.

Victor FormanNiels Bjerg-JensenJane D DyekjærBirger Lindberg MøllerIrini Pateraki

Published in: Microbial cell factories (2018)

As demonstrated for CYP76AH15, site-directed mutagenesis of SRS regions of plant CYPs may be an efficient and targeted approach to increase the performance of these enzymes. Although in this work we have managed to achieve higher efficiency and specificity of the first CYP of the pathway, further work is necessary in order to increase the overall production of forskolin in yeast cells.

Keyphrases

cell wall
induced apoptosis
saccharomyces cerevisiae
cell cycle arrest
crispr cas
structural basis
cancer therapy
oxidative stress
cell death
signaling pathway
endoplasmic reticulum stress
drug delivery