Deciphering molecular interactions by proximity labeling.
Wei QinKelvin F ChoPeter E CavanaghAlice Y TingPublished in: Nature methods (2021)
Many biological processes are executed and regulated through the molecular interactions of proteins and nucleic acids. Proximity labeling (PL) is a technology for tagging the endogenous interaction partners of specific protein 'baits', via genetic fusion to promiscuous enzymes that catalyze the generation of diffusible reactive species in living cells. Tagged molecules that interact with baits can then be enriched and identified by mass spectrometry or nucleic acid sequencing. Here we review the development of PL technologies and highlight studies that have applied PL to the discovery and analysis of molecular interactions. In particular, we focus on the use of PL for mapping protein-protein, protein-RNA and protein-DNA interactions in living cells and organisms.
Keyphrases
- living cells
- protein protein
- single molecule
- small molecule
- nucleic acid
- fluorescent probe
- mass spectrometry
- high resolution
- binding protein
- amino acid
- liquid chromatography
- high throughput
- multidrug resistant
- copy number
- hepatitis c virus
- single cell
- ms ms
- human immunodeficiency virus
- circulating tumor
- hiv testing
- case control