Anti-Osteoclastogenic and Antibacterial Effects of Chlorinated Polyketides from the Beibu Gulf Coral-Derived Fungus Aspergillus unguis GXIMD 02505.
Yanting ZhangZhichao LiBingyao HuangKai LiuShuai PengXinming LiuChenghai GaoYong-Hong LiuYanhui TanXiao-Wei LuoPublished in: Marine drugs (2022)
One new depsidone derivative, aspergillusidone H ( 3 ), along with seven known biosynthetically related chlorinated polyketides, were obtained from the Beibu Gulf coral-derived fungus Aspergillus unguis GXIMD 02505. Their structures were determined by comprehensive physicochemical and spectroscopic data interpretation. Notably, the X-ray crystal structure of 2 and the previously unknown absolute configuration of 8, assigned by ECD calculations, are described here for the first time. Compounds 1 - 5 , 7 and 8 exhibited inhibition of lipopolysaccharide (LPS)-induced NF-κB in RAW 264.7 macrophages at 20 μM. In addition, the two potent inhibitors ( 2 and 7 ) dose-dependently suppressed RANKL-induced osteoclast differentiation without any evidence of cytotoxicity in bone marrow macrophages cells (BMMs). This is the first report of osteoclastogenesis inhibitory activity for the metabolites of these kinds. Besides, compounds 1 , 2 , 4 , and 6 - 8 showed inhibitory activity against marine biofilm-forming bacteria, methicillin-resistant Staphylococcus aureus , Microbulbifer variabilis , Marinobacterium jannaschii , and Vibrio pelagius , with their MIC values ranging from 2 to 64 μg/mL. These findings provide a basis for further development of chlorinated polyketides as potential inhibitors of osteoclast differentiation and/or for use as anti-fouling agents.
Keyphrases
- lps induced
- methicillin resistant staphylococcus aureus
- inflammatory response
- staphylococcus aureus
- bone loss
- bone marrow
- polycyclic aromatic hydrocarbons
- gas chromatography
- induced apoptosis
- high resolution
- biofilm formation
- mesenchymal stem cells
- pseudomonas aeruginosa
- cell cycle arrest
- molecular docking
- high glucose
- ms ms
- mass spectrometry
- candida albicans
- molecular dynamics
- molecular dynamics simulations
- electronic health record
- diabetic rats
- anti inflammatory
- cell wall
- toll like receptor
- drug induced
- computed tomography
- magnetic resonance imaging
- endoplasmic reticulum stress
- oxidative stress
- escherichia coli
- nuclear factor
- risk assessment
- tandem mass spectrometry
- dual energy
- signaling pathway
- human health
- cystic fibrosis
- cell death
- silver nanoparticles
- magnetic resonance