Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris.
Mikako TachiokaNaohisa SugimotoAkihiko NakamuraNaoki SunagawaTakuya IshidaTaku UchiyamaKiyohiko IgarashiMasahiro SamejimaPublished in: Biotechnology for biofuels (2016)
We present a rapid and convenient random mutagenesis method that does not require laborious steps such as ligation, cloning, and synthesis of specific primers. This method was successfully applied to the protein expression system in P. pastoris.