Ascorbic Acid-Loaded Apoferritin-Assisted Carbon Dot-MnO 2 Nanocomposites for the Selective and Sensitive Detection of Trypsin.
Qingqing TanRuirui ZhangWeisu KongFeng-Li QuLimin LuPublished in: ACS applied bio materials (2018)
A novel "turn-on" fluorescent trypsin detection platform dependant on carbon dot-MnO 2 (CD-MnO 2 ) nanocomposites and ascorbic acid-loaded apoferritin (APOAA) was fabricated. The detection mechanism relied on trypsin-catalyzed enzymolysis of APOAA, which released ascorbic acid (AA) as a reducing agent to disintegrate the MnO 2 nanosheets, causing the recovery of the fluorescence of CDs. An excellent performance and high sensitivity of trypsin determination were observed with a detection limit (LOD) of 0.3411 ng/mL. This work provides us with a unique strategy for trypsin detection in human serum samples, which reveals the potential applications in clinical detection.
Keyphrases
- loop mediated isothermal amplification
- sensitive detection
- quantum dots
- label free
- real time pcr
- drug delivery
- reduced graphene oxide
- risk assessment
- cancer therapy
- room temperature
- energy transfer
- wound healing
- single molecule
- carbon nanotubes
- highly efficient
- solid phase extraction
- human health
- tandem mass spectrometry