A 20-year experience of ocular herpes virus detection using immunofluorescence and polymerase chain reaction.
Gita SatpathyHimanshu Sekhar BeheraAnjana SharmaAbhisek K MishraDeepanshi MishraNamrata SharmaRadhika TandonTushar AgarwalJeewan S TitiyalPublished in: Clinical & experimental optometry (2018)
Efficacy and accuracy of the polymerase chain reaction assay was greater compared to the immunofluorescence assay for detection of herpes virus in corneal scrapings/corneal grafts of suspected herpetic keratitis patients. Although the immunofluorescence assay is a rapid test for the detection of herpes virus in suspected herpetic keratitis patients, a combination of polymerase chain reaction with immunofluorescence assay will provide higher reliable and accurate results.