A 20-year experience of ocular herpes virus detection using immunofluorescence and polymerase chain reaction.

Gita SatpathyHimanshu Sekhar BeheraAnjana SharmaAbhisek K MishraDeepanshi MishraNamrata SharmaRadhika TandonTushar AgarwalJeewan S Titiyal

Published in: Clinical & experimental optometry (2018)

Efficacy and accuracy of the polymerase chain reaction assay was greater compared to the immunofluorescence assay for detection of herpes virus in corneal scrapings/corneal grafts of suspected herpetic keratitis patients. Although the immunofluorescence assay is a rapid test for the detection of herpes virus in suspected herpetic keratitis patients, a combination of polymerase chain reaction with immunofluorescence assay will provide higher reliable and accurate results.

Keyphrases

end stage renal disease
high throughput
loop mediated isothermal amplification
ejection fraction
chronic kidney disease
newly diagnosed
prognostic factors
peritoneal dialysis
pulmonary embolism
patient reported outcomes
label free