Identification and quantification of nine compounds in fangwen jiuwei decoction by liquid chromatography-mass spectrometry.
Jing ZhuYang YangLing-Yi XinShi-Yu WanNa HeHang-Tian WangXi-Yu ChenQuan-Xi MeiGuang-Jun FengQin-Hua ChenGuang-Yi YangPublished in: Journal of separation science (2023)
Fangwen Jiuwei Decoction is a traditional Chinese medicine preparation for the treatment of pneumonia developed by Shenzhen Bao'an Chinese Medicine Hospital, which shows remarkable clinical responses. Qualitative and quantitative analyses of the main active compounds are crucial for the quality control of traditional Chinese medicine prescription in clinical application. In this study, we identified nine active compounds essential for the pharmacological effects of Fangwen Jiuwei Decoction based on the analysis of the Network Pharmacology and relevant literature. Moreover, these compounds can interact with several crucial drug targets in pneumonia based on molecular docking. We applied HPLC-MS/MS method was established these nine active ingredients' qualitative and quantitative detections. The possible cleavage pathways of nine active components were determined based on secondary ions mass spectrometry. The results of HPLC-MS/MS were further validated, which show a satisfactory correlation coefficient (r>0.99), recovery rate (≥93.31%), repeatability rate (≤5.62%), stability (≤7.95%), intra-day precision (≤6.68%), and inter-day precision (≤9.78%). The limit of detection was as low as 0.01ng/mL. In this study, we estabilished an HPLC-MS/MS method to qualitatively and quantitatively analyze the chemical components in the Fangwen Jiuwei Decoction extract. This article is protected by copyright. All rights reserved.
Keyphrases
- ms ms
- mass spectrometry
- liquid chromatography
- high performance liquid chromatography
- molecular docking
- tandem mass spectrometry
- simultaneous determination
- liquid chromatography tandem mass spectrometry
- high resolution
- quality control
- systematic review
- ultra high performance liquid chromatography
- solid phase extraction
- healthcare
- high resolution mass spectrometry
- gas chromatography
- oxidative stress
- magnetic resonance imaging
- emergency department
- molecular dynamics simulations
- transcription factor
- computed tomography
- capillary electrophoresis
- adverse drug
- combination therapy
- network analysis