pH-dependent complex formation with TAR RNA and DNA: application towards logic gates.
Rakesh PaulRaj PaulDebasish DuttaJyotirmayee DashPublished in: The Analyst (2024)
Nucleic acid-based logic gates have shown great potential in biotechnology, medicine as well as diagnostics. Herein, we have constructed pH-responsive logic devices by utilizing HIV-1 TAR hairpins in combination with a thiazole peptide that exhibits turn-on fluorescence upon interacting with TAR RNA or DNA. Based on this, INHIBIT-AND and YES-INHIBIT-AND logic gates were constructed in parallel. The pH alteration leads to conformational changes of the hairpin structure, enabling the construction of a multi-reset reusable logic system which could be developed for in vitro sensing of the HIV-1 viral RNA.
Keyphrases
- nucleic acid
- single molecule
- antiretroviral therapy
- hiv positive
- hiv infected
- human immunodeficiency virus
- hiv testing
- hepatitis c virus
- hiv aids
- wastewater treatment
- men who have sex with men
- sars cov
- circulating tumor
- molecular dynamics
- cell free
- risk assessment
- south africa
- living cells
- fluorescent probe
- climate change