Piranhea trifoliata extracts ameliorate muscular decline in Drosophila melanogaster exposed to Paraquat.
Letícia P de AlencarLorena L da CostaDouglas R LisboaJadyellen R SilvaStephanie F SantosMayara P PereiraKlenicy K de Lima YamaguchiAnderson de Oliveira SouzaPublished in: Archives of insect biochemistry and physiology (2022)
In this study, we have demonstrated, for the first time, the muscular protective effects of Piranhea trifoliata bark extract against Paraquat (PQ)-induced oxidative stress in Drosophila melanogaster. Exposure of D. melanogaster (Canton Special) to PQ caused oxidative stress, as evidenced by protein carbonyl and elevated acetylcholinesterase (AChE) activity levels. However, a diet supplemented with the P. trifoliata extracts (0.1 mg/ml) for 10 days ameliorates protein carbonyl levels and enzymatic activities of AChE and citrate synthase to prevent PQ damage. Also, P. trifoliata bark extracts showed in phytochemical assays the presence of phenols, at 46.06 mg EAG/g extract of total phenolic compounds, and a 40% 2,2-diphenyl-1-picryl-hydrazyl scavenging effect. The study showed the muscular protective function of the P. trifoliata extracts in D. melanogaster exposed to PQ. On the basis of the results, we contemplate that the bark of P. trifoliata might prevent and ameliorate human diseases caused by oxidative stress. The muscular action of the P. trifoliata extract can be attributed to the antioxidant constituents, while the precise mechanism of its action needs further investigation.
Keyphrases
- oxidative stress
- drosophila melanogaster
- resistance training
- dna damage
- diabetic rats
- ischemia reperfusion injury
- induced apoptosis
- hydrogen peroxide
- anti inflammatory
- endothelial cells
- physical activity
- protein protein
- nitric oxide
- weight loss
- small molecule
- body composition
- high throughput
- single cell
- amino acid
- induced pluripotent stem cells