Dot Immunobinding Assay for the Rapid Serodetection of Scedosporium / Lomentospora in Cystic Fibrosis Patients.
Leire Martin-SoutoAitziber AntoranMaialen AreitioLeire Aparicio-FernandezMaría Teresa Martin-GómezRoberto FernandezEgoitz AstigarragaGabriel Barreda-GómezCarsten SchwarzVolker RickertsFernando L HernandoAitor RementeriaIdoia BuldainAndoni Ramirez-GarciaPublished in: Journal of fungi (Basel, Switzerland) (2023)
The detection of Scedosporium / Lomentospora is still based on non-standardized low-sensitivity culture procedures. This fact is particularly worrying in patients with cystic fibrosis (CF), where these fungi are the second most common filamentous fungi isolated, because a poor and delayed diagnosis can worsen the prognosis of the disease. To contribute to the discovery of new diagnostic strategies, a rapid serological dot immunobinding assay (DIA) that allows the detection of serum IgG against Scedosporium / Lomentospora in less than 15 min was developed. A crude protein extract from the conidia and hyphae of Scedosporium boydii was employed as a fungal antigen. The DIA was evaluated using 303 CF serum samples (162 patients) grouped according to the detection of Scedosporium / Lomentospora in the respiratory sample by culture, obtaining a sensitivity and specificity of 90.48% and 79.30%, respectively; positive and negative predictive values of 54.81% and 96.77%, and an efficiency of 81.72%. The clinical factors associated with the results were also studied using a univariate and a multivariate analysis, which showed that Scedosporium / Lomentospora positive sputum, elevated anti- Aspergillus serum IgG and chronic Pseudomonas aeruginosa infection were significantly associated with a positive result in DIA, while Staphylococcus aureus positive sputum showed a negative association. In conclusion, the test developed can offer a complementary, rapid, simple and sensitive method to contribute to the diagnosis of Scedosporium / Lomentospora in patients with CF.
Keyphrases
- cystic fibrosis
- pseudomonas aeruginosa
- end stage renal disease
- loop mediated isothermal amplification
- newly diagnosed
- ejection fraction
- staphylococcus aureus
- chronic kidney disease
- prognostic factors
- mycobacterium tuberculosis
- oxidative stress
- escherichia coli
- chronic obstructive pulmonary disease
- lung function
- multidrug resistant
- pulmonary tuberculosis
- label free
- acinetobacter baumannii
- binding protein
- protein protein
- sensitive detection
- solid state