Empirical identification and validation of tumor-targeting T cell receptors from circulation using autologous pancreatic tumor organoids.
Qingda MengShanshan XieG Kenneth GrayMohammad H DezfulianWeilin LiLing HuangDipikaa AkshinthalaElizabeth FerrerCatherine ConahanSofia Perea Del PinoJoseph GrossmanStephen J ElledgeManuel HidalgoSenthil K MuthuswamyPublished in: Journal for immunotherapy of cancer (2022)
We report a platform for expanding tumor-targeting T cells from the peripheral blood of patients with pancreatic cancer. We identify the NKG2A-HLA-E axis as a potentially important checkpoint for CD8 +T cells for pancreatic cancer. Lastly, we demonstrate empirical identification of tumor-targeting TCRs that can be used for TCR-therapeutics.