Deciphering the minimal quantity of mouse primary cells to undergo nephrogenesis ex vivo.
Aleksandra Rak-RaszewskaGanna ReintFabienne GeigerFlorence NaillatSeppo J VainioPublished in: Developmental dynamics : an official publication of the American Association of Anatomists (2021)
We show that a significant decrease of cells used to generate renal mouse organoids in a dissociation/re-aggregation assay, does not interfere with development, and goes toward 3Rs. This enables generation of more experimental samples with one mouse litter, limiting the number of animals used for studies.