Pyruvate alleviates high glucose-induced endoplasmic reticulum stress and apoptosis in HK-2 cells.
Xiao Meng ZhangYi Zhen WangJin Dong TongXu Chao NingFang Qiang ZhouXiu Hong YangHui Min JinPublished in: FEBS open bio (2020)
Endoplasmic reticulum (ER) stress plays a critical role in the development of diabetic nephropathy (DN). We previously demonstrated that pyruvate (Pyr)-enriched oral rehydration solution improved glucometabolic disorders and ameliorated DN outcome in db/db mice. Here, we investigated the effects of Pyr on high glucose-induced ER stress and apoptosis in HK-2 cells. Our results suggest that high glucose can induce reactive oxygen species production, apoptosis and ER stress in HK-2 cells, and that Pyr treatment can ameliorate these effects and restore the expression of key proteins involved in ER stress. Thus, Pyr may have potential for the development of novel strategies for the prevention and treatment of clinical DN.
Keyphrases
- high glucose
- endoplasmic reticulum stress
- induced apoptosis
- cell cycle arrest
- endothelial cells
- cell death
- oxidative stress
- pi k akt
- diabetic nephropathy
- endoplasmic reticulum
- reactive oxygen species
- signaling pathway
- metabolic syndrome
- mouse model
- risk assessment
- mass spectrometry
- replacement therapy
- adipose tissue
- smoking cessation
- high resolution