Quaternary Ammonium Salt-Functionalized Tetraphenylethene Derivative Boosts Electrochemiluminescence for Highly Sensitive Aqueous-Phase Biosensing.
Wenxin LvQiaoting YangQian LiHaiyin LiFeng LiPublished in: Analytical chemistry (2020)
Aggregation induced emission active compounds (AIEgens) have appeared as a new kind of electrochemiluminescence (ECL) emitters due to their bright emission in the aggregated state but lack functional groups. Herein, we report a quaternary ammonium salt groups-functionalized AIEgen (QAU-1) and discover that coating QAU-1 on the indium tin oxide (ITO) surface (QAU/ITO) enabled QAU-1 to display significant cathodic ECL emission compared with that of QAU-1 in the dissolved state. Inspired by this, we applied QAU-1 as emitters to develop a novel ECL biosensor (Fc-DNA/QAU/ITO) through electrostatic attraction between QAU/ITO and a ferrocene-labeled ssDNA (Fc-DNA), and the developed biosensor was employed to detect bleomycin (BLM) with high sensitivity based on the target-initiated specific cleavage and subsequent removal of Fc molecules from the electrode. We envision this work will open up a new avenue to development of high-performance ECL biosensors, which will display a significant potential application in the field of analysis.
Keyphrases
- sensitive detection
- quantum dots
- label free
- ionic liquid
- circulating tumor
- energy transfer
- cell free
- solid state
- single molecule
- gold nanoparticles
- light emitting
- minimally invasive
- nucleic acid
- risk assessment
- dna binding
- human health
- oxide nanoparticles
- high resolution
- pulmonary fibrosis
- mass spectrometry
- transcription factor
- fluorescent probe
- climate change