Flavonoid Metabolites in Human Urine during Blueberry Anthocyanin Intake.
Wilhelmina KaltJane E McDonaldYan LiuSherry A E FillmorePublished in: Journal of agricultural and food chemistry (2017)
The human health benefits of anthocyanins (Anc) and other flavonoids are widely recognized. However, the flavonoid-based urinary metabolites arising in vivo after Anc intake are not well described. Human (n = 17) urine was collected while blueberry juice (BJ) was consumed daily for 28 days and once after a 7 day washout. MS/MS scanning of 664 urine samples for 18 parent Anc (PAnc) and 42 predicted Anc metabolites (AncM) yielded 371 products (i.e., MS/MS × retention time (RT)). Flavonoid-based AncM, which were likely underestimated, were almost 20 times more abundant than PAnc. Together, PAnc and AncM accounted for about 1% of the daily Anc dose. Aglycone forms were >94% of the total. Cluster analysis of the 371 Anc identified about 55 major Anc that contributed about 80% to the total Anc. The abundance of flavonoid-based Anc-derived products in the gastrointestinal tract could contribute to the health benefits of Anc-rich berries.
Keyphrases
- ms ms
- human health
- endothelial cells
- risk assessment
- healthcare
- public health
- mental health
- high resolution
- mass spectrometry
- climate change
- microbial community
- high performance liquid chromatography
- social media
- antibiotic resistance genes
- ultra high performance liquid chromatography
- tandem mass spectrometry
- wastewater treatment
- anaerobic digestion