Gliogenic Potential of Single Pallial Radial Glial Cells in Lower Cortical Layers.
Ana Cristina Ojalvo-SanzLaura Lopez-MascaraquePublished in: Cells (2021)
During embryonic development, progenitor cells are progressively restricted in their potential to generate different neural cells. A specific progenitor cell type, the radial glial cells, divides symmetrically and then asymmetrically to produce neurons, astrocytes, oligodendrocytes, and NG2-glia in the cerebral cortex. However, the potential of individual progenitors to form glial lineages remains poorly understood. To further investigate the cell progeny of single pallial GFAP-expressing progenitors, we used the in vivo genetic lineage-tracing method, the UbC-(GFAP-PB)-StarTrack. After targeting those progenitors in embryonic mice brains, we tracked their adult glial progeny in lower cortical layers. Clonal analyses revealed the presence of clones containing sibling cells of either a glial cell type (uniform clones) or two different glial cell types (mixed clones). Further, the clonal size and rostro-caudal cell dispersion of sibling cells differed depending on the cell type. We concluded that pallial E14 neural progenitors are a heterogeneous cell population with respect to which glial cell type they produce, as well as the clonal size of their cell progeny.
Keyphrases
- induced apoptosis
- single cell
- cell cycle arrest
- cell therapy
- neuropathic pain
- endoplasmic reticulum stress
- oxidative stress
- type diabetes
- metabolic syndrome
- stem cells
- spinal cord
- dna methylation
- skeletal muscle
- gene expression
- risk assessment
- cell proliferation
- genome wide
- adipose tissue
- insulin resistance
- brain injury
- pi k akt
- high fat diet induced