Shedding Light on Heavy Metal Contamination: Fluorescein-Based Chemosensor for Selective Detection of Hg 2+ in Water.
Maksim N ZavalishinAleksei N KiselevAlexandra K IsagulievaAnna V ShibaevaVladimir A KuzminVladimir N MorozovEugene A ZevakinUlyana A PetrovaAlina A KnyazevaAlexey V EroshinYuriy A ZhabanovGeorge A GamovPublished in: International journal of molecular sciences (2024)
This article discusses the design and analysis of a new chemical chemosensor for detecting mercury(II) ions. The chemosensor is a hydrazone made from 4-methylthiazole-5-carbaldehyde and fluorescein hydrazide. The structure of the chemosensor was confirmed using various methods, including nuclear magnetic resonance spectroscopy, infrared spectroscopy with Fourier transformation, mass spectroscopy, and quantum chemical calculations. The sensor's ability in the highly selective and sensitive discovery of Hg 2+ ions in water was demonstrated. The detection limit for mercury(II) ions was determined to be 0.23 µM. The new chemosensor was also used to detect Hg 2+ ions in real samples and living cells using fluorescence spectroscopy. Chemosensor 1 and its complex with Hg 2+ demonstrate a significant tendency to enter and accumulate in cells even at very low concentrations.
Keyphrases
- aqueous solution
- single molecule
- living cells
- fluorescent probe
- heavy metals
- molecular dynamics
- high resolution
- risk assessment
- small molecule
- induced apoptosis
- loop mediated isothermal amplification
- label free
- health risk
- drinking water
- cell cycle arrest
- molecular dynamics simulations
- solid state
- mass spectrometry
- human health
- anaerobic digestion