Imaging Keratan Sulfate in Ocular Tissue Sections by Immunofluorescence Microscopy and LA-ICP-MS.
Burak BoyrazJessica SaatzInga-Marie PompösMichel GadJens DerneddeAnna-Karina B MaierOren MoscovitzPeter H SeebergerHeike TraubRudolf TauberPublished in: ACS applied bio materials (2022)
Carbohydrate-specific antibodies can serve as valuable tools to monitor alterations in the extracellular matrix resulting from pathologies. Here, the keratan sulfate-specific monoclonal antibody MZ15 was characterized in more detail by immunofluorescence microscopy as well as laser ablation ICP-MS using tissue cryosections and paraffin-embedded samples. Pretreatment with keratanase II prevented staining of samples and therefore demonstrated efficient enzymatic keratan sulfate degradation. Random fluorescent labeling and site-directed introduction of a metal cage into MZ15 were successful and allowed for a highly sensitive detection of the keratan sulfate landscape in the corneal stroma from rats and human tissue.
Keyphrases
- sensitive detection
- extracellular matrix
- high resolution
- monoclonal antibody
- quantum dots
- mass spectrometry
- optical coherence tomography
- high speed
- multiple sclerosis
- single molecule
- ms ms
- endothelial cells
- label free
- high throughput
- hydrogen peroxide
- nitric oxide
- single cell
- atrial fibrillation
- optic nerve
- photodynamic therapy
- loop mediated isothermal amplification
- electron microscopy
- fluorescent probe