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Understanding Secondary Structures of Silk Materials via Micro- and Nano-Infrared Spectroscopies.

Jiajia ZhongYawen LiuJing RenYuzhao TangZeming QiXiaojie ZhouXin ChenZhengzhong ShaoMin ChenDavid Lee KaplanShengjie Ling
Published in: ACS biomaterials science & engineering (2019)
The secondary structures (also termed conformations) of silk fibroin (SF) in animal silk fibers and regenerated SF materials are critical in determining mechanical performance and function of the materials. In order to understand the structure-mechanics-function relationships of silk materials, a variety of advanced infrared spectroscopic techniques, such as micro-infrared spectroscopies (micro-IR spectroscopies for short), synchrotron micro-IR spectroscopy, and nano-infrared spectroscopies (nano-IR spectroscopies for short), have been used to determine the conformations of SF in silk materials. These IR spectroscopic methods provide a useful toolkit to understand conformations and conformational transitions of SF in various silk materials with spatial resolution from the nano-scale to the micro-scale. In this Review, we first summarize progress in understanding the structure and structure-mechanics relationships of silk materials. We then discuss the state-of-the-art micro- and nano-IR spectroscopic techniques used for silk materials characterization. We also provide a systematic discussion of the strategies to collect high-quality spectra and the methods to analyze these spectra. Finally, we demonstrate the challenges and directions for future exploration of silk-based materials with IR spectroscopies.
Keyphrases
  • tissue engineering
  • wound healing
  • molecular docking
  • high resolution
  • single molecule