Mapping fluorescence resonance energy transfer parameters of a bifunctional agent using time-domain fluorescence diffuse optical tomography.
Weirong MoNayan J PatelYihui ChenRavindra PandeyUlas SunarPublished in: Journal of biophotonics (2020)
We present a method to map fluorescence resonance energy transfer (FRET) parameters of a bifunctional photodynamic therapy agent, (2-[1-hexyloxyethyl]-2-devinyl pyropheophorbide-a)-cyanine dye (HPPH-CD) conjugate, which consists of a photosensitizer (HPPH) and a fluorescent agent CD. We utilized time-domain fluorescence diffuse optical tomography, the normalized Born ratio model in the Fourier-domain, and an iterative algorithm to map depth-resolved spatial heterogeneities of FRET parameters. Our results exhibited depth-resolved changes of fluorophore's lifetime and the distance maps due to FRET between HPPH and CD. Our model suggests a potential approach of using FRET parameters to monitor efficacies of multifunctional photodynamic therapy agents in deep tissue.
Keyphrases
- energy transfer
- photodynamic therapy
- quantum dots
- high resolution
- fluorescence imaging
- highly efficient
- drug delivery
- optical coherence tomography
- machine learning
- low grade
- nk cells
- cancer therapy
- metal organic framework
- high speed
- living cells
- computed tomography
- fluorescent probe
- single molecule
- deep learning
- label free
- human health
- electron microscopy