Characterization of spironolactone and metabolites derivatized using Girard's reagent P using mass spectrometry and ion mobility spectrometry.
Stephanie M JonesKaylie I Kirkwood-DonelsonGeorgia M AlexanderLalith PereraSerena M DudekAlan K JarmuschPublished in: Rapid communications in mass spectrometry : RCM (2024)
GP derivatization was proven to have advantageous mass spectral performance (e.g., limiting in-source fragmentation, enhancing signals, and eliminating isobaric analytes) for spironolactone and its metabolites. This work and the detailed characterization using ultra-high-performance liquid chromatography-high-resolution tandem mass spectrometry (UHPLC-HRMS/MS) and IMS serve as the foundation for future developments in reaction optimization and/or quantitative assay development.
Keyphrases
- tandem mass spectrometry
- ultra high performance liquid chromatography
- high resolution
- mass spectrometry
- liquid chromatography
- gas chromatography
- high resolution mass spectrometry
- ms ms
- high performance liquid chromatography
- simultaneous determination
- solid phase extraction
- liquid chromatography tandem mass spectrometry
- current status
- capillary electrophoresis
- gas chromatography mass spectrometry
- multiple sclerosis
- optical coherence tomography
- high throughput
- magnetic resonance imaging
- high speed