Cardiovascular benefits of Eruca sativa mill. Defatted seed meal extract: Potential role of hydrogen sulfide.
Lara TestaiEleonora PagnottaEugenia PiragineLorenzo FloriValentina CitiAlma MartelliDi Cesare Mannelli LorenzoCarla GhelardiniRoberto MatteoSerafino SurianoAntonio TroccoliNicola PecchioniVincenzo CalderonePublished in: Phytotherapy research : PTR (2022)
Eruca sativa Mill. is an edible plant belonging to the Brassicaceae botanical family with a long story as a medicinal material, mainly linked to the presence of glucoerucin. One of the main products of this glucosinolate is erucin, a biologicallly active isothiocyanate recently recognized as a hydrogen sulfide (H 2 S) donor. In this work, an Eruca sativa extract has been obtained from a defatted seed meal (DSM), achieving a powder rich in thiofunctionalized glucosinolates, glucoerucin, and glucoraphanin, accounting for 95% and 5% of the total glucosinolate content (17% on a dry weight basis), associated with 13 identified phenolic acids and flavonoids accounting for 2.5%. In a cell-free model, Eruca sativa DSM extract slowly released H 2 S. Moreover, this extract promoted significant hypotensive effects in hypertensive rats, and evoked dose-dependent cardioprotection in in vivo model of acute myocardial infarct, obtained through a reversible coronary occlusion. This latter effect was sensitive to blockers of mitochondrial KATP and Kv7.4 potassium channels, suggesting a potential role of these mitochondrial channels in the protective effects of Eruca sativa DSM extract. Accordingly, Eruca sativa DSM extract reduced calcium uptake and apoptotic cell death in isolated cardiac mitochondria. Taken together, these results demonstrate that Eruca sativa DSM extract is endowed with an interesting nutraceutical profile on the cardiovascular system due to, at least in part, its H 2 S releasing properties. These results pave the way for future investigations on active metabolites.
Keyphrases
- oxidative stress
- cell death
- anti inflammatory
- cell free
- magnetic resonance imaging
- left ventricular
- physical activity
- body mass index
- acute myocardial infarction
- computed tomography
- magnetic resonance
- weight loss
- cell proliferation
- extracorporeal membrane oxygenation
- respiratory failure
- current status
- climate change
- drug induced
- mechanical ventilation