Whole-Genome Sequencing of Candida haemulonii species complex from Brazil and the United States: genetic diversity and antifungal susceptibility.
Dality Keffelen Barros de RodriguesShawn R LockhartElizabeth L BerkowLalitha GadeLucas Xavier BonfiettiViviane Mazo Fávero GimenesLuciana da Silva RuizMilena Bronze MacioniMarcia de Souza Carvalho MelhemPublished in: Medical mycology (2023)
Candida haemulonii complex species can be multidrug-resistant (MDR) and cause infections such as candidemia. This study determined the genetic relationship between isolates from Brazil and the United States through whole-genome sequencing and performed antifungal susceptibility testing to investigate drug resistance. Contrary to what is widely described, most isolates were susceptible to azoles. However, an atypical susceptibility profile was found in 50% of C. pseudohaemulonii strains including resistance to the three echinocandins. Isolates from both countries formed distinct clusters with wide genetic diversity. Isolates from three hospitals in Brazil were clonal and involved in candidemia cases, pointing to the importance of improving hospital infection control measures and molecular identification.
Keyphrases
- genetic diversity
- candida albicans
- multidrug resistant
- biofilm formation
- healthcare
- drug resistant
- escherichia coli
- gram negative
- acinetobacter baumannii
- genome wide
- emergency department
- klebsiella pneumoniae
- gene expression
- dna methylation
- staphylococcus aureus
- cystic fibrosis
- copy number
- drug induced
- bioinformatics analysis