Characterization and mechanisms of radioresistant lung squamous cell carcinoma cell lines.
Lifang PanQiong WuYuqing WangShenglin MaShi-Rong ZhangPublished in: Thoracic cancer (2023)
After fractionated irradiation (total dose of 60 Gy), radioresistant cells had decreased radiosensitivity, increased G0/G1 phase arrest, enhanced DNA damage repair ability, and through the ATM/CHK2 and DNA-PKcs/Ku70 pathways regulated double strands break. The upregulated differential genes in radioresistant cell lines were mainly enriched in biological pathways such as cell migration and extracellular matrix (ECM)-receptor interaction. In vivo verification of decreased radiosensitivity of radioresistant cells CONCLUSIONS: Radioresistant LUSC cell lines were established by fractional radiotherapy, which regulates IR-induced DNA damage repair through ATM/CHK2 and DNA-PKcs/Ku70. Tandem Mass Tags (TMT) quantitative proteomics found that the biological process pathway of cell migration and ECM-receptor interaction are upregulated in LUSC radioresistant cells.
Keyphrases
- dna damage
- cell migration
- induced apoptosis
- extracellular matrix
- squamous cell carcinoma
- cell cycle arrest
- dna repair
- oxidative stress
- dna damage response
- early stage
- circulating tumor
- radiation therapy
- gene expression
- cell death
- single molecule
- locally advanced
- signaling pathway
- cell proliferation
- stress induced
- lymph node metastasis
- endothelial cells
- genome wide identification