Detection of CTX-M-15 Extended-Spectrum β-Lactamases Producing Escherichia coli Isolates from Colostrum and Faeces of Newborn Dairy Calves in China.
Zhiyuan HeSirui YangYulin MaShuyuan ZhangZhi-Jun CaoPublished in: Pathogens (Basel, Switzerland) (2021)
Newborn dairy calves are often colonized by multidrug-resistant (MDR) extended-spectrum β-Lactamase producing Escherichia coli (ESBL-EC), which pose significant risks to global healthcare. As the first meal of calves, the role of dairy colostrum as a potential source of MDR-E. coli has not been well-studied. Here, we report on similar antibiotic resistance patterns of E. coli strains, isolated from colostrum fed to dairy calves and their faeces. Four ESBL-EC strains from colostrum and faeces of newborn dairy calves were isolated by double-disc synergy testing and multiplex PCR. Strikingly, isolates from colostrum or faeces were found to have similar MDR profiles, showing a high resistance to cephalosporins and other conventional antibiotics. In addition, coexistence of blaCTX-M-15 and blaTEM-171 was detected on a self-transferable plasmid with a typical IncHI2 backbone. To the best of our knowledge, this is the first case reporting on ESBL-EC strains carrying blaCTX-M-15 and blaTEM-171 genes, and isolated from faeces and the colostrum stock fed to the dairy calves.
Keyphrases
- escherichia coli
- klebsiella pneumoniae
- multidrug resistant
- human milk
- healthcare
- drug resistant
- biofilm formation
- gram negative
- acinetobacter baumannii
- low birth weight
- emergency department
- real time pcr
- risk assessment
- crispr cas
- transcription factor
- dna methylation
- pseudomonas aeruginosa
- solid state
- preterm birth
- health insurance
- health information
- single cell
- electronic health record