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DNA-Mediated Proximity-Based Assembly Circuit for Actuation of Biochemical Reactions.

Sung Won OhAdriana PereiraTing ZhangTianran LiAriel LaneJinglin Fu
Published in: Angewandte Chemie (International ed. in English) (2018)
Smart nanodevices that integrate molecular recognition and signal production hold great promise for the point-of-care (POC) diagnostic applications. Herein, the development of a DNA-mediated proximity assembly of biochemical reactions, which was capable of sensing various bio-targets and reporting easy-to-read signals is reported. The circuit was composed of a DNA hairpin-locked catalytic cofactor with inhibited activity. Specific molecular inputs can trigger a conformational switch of the DNA locks through the mechanisms of toehold displacement and aptamer switching, exposing an active cofactor. The subsequent assembly of an enzyme/cofactor pair actuated a reaction to produce colorimetric or fluorescence signals for detecting target molecules. The developed system could be potentially applied to smart biosensing in molecular diagnostics and POC tests.
Keyphrases
  • single molecule
  • living cells
  • gold nanoparticles
  • circulating tumor
  • sensitive detection
  • cell free
  • molecular dynamics simulations
  • big data
  • nitric oxide
  • magnetic nanoparticles